Impact of Cell-free Supernatant of Lactic Acid Bacteria on Putrescine and Other Polyamine Formation by Foodborne Pathogens in Ornithine Decarboxylase Broth


ÖZOĞUL F. , TABANELLI G., Toy N. , GARDINI F.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.63, ss.5828-5835, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 63 Konu: 24
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/acs.jafc.5b02410
  • Dergi Adı: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Sayfa Sayısı: ss.5828-5835

Özet

Conversion of omithine to putrescine by Salmonella Paratyphi A, Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli was investigated in ornithine decarboxylase broth (ODB) using cell-free supernatants (CFSs) obtained from Leuconostoc mesenterodies subsp. cremoris, Pediococcus acidilactici, Lactococcus lactis Subsp. lactis, Streptococcus thermophilus. Two groups of cell-free supernatants (25 or 50%) and control (only ODB) were prepared to investigate putrescine (PUT) and other polyamine formation by foodborne pathogens (FBPs). Significant differences (p<0.05) were observed among the species, for each amine. All of the CFSs reduce the formation of PUT by >= 65%, The production of cadaverine (CAD) was scarcely affected by the presence of CFSs, with the exception of the samples inoculated with L. monocytogenes. The variation in polyamine was found with respect to the control samples. Spermidine (SPD) was produced in lower amount in ninny,samples, especially in Gram-negative FBPs, whereas spermine (SPN) increased drastically in the major part of the samples concerning the control. Histamine (HIS) was characterized by a marked concentration decrease in all of the Samples, and tyramine (TYR) was accumulated in very low concentrations in the controls. Therefore, the ability of bacteria to Produce certain biogenic amines such, as HIS, TYR, pur, and CAD has been studied to assess their risk and prevent their formation in food products. The results obtained from this study concluded that the lactic acid bacteria (LAB), strains with non-decarboxylage activity are capable of avoiding or limiting biogenic amine formation by FBP.