Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)

Atis I., Can E., Celiktas N., HATİPOĞLU R.

JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT, cilt.11, sa.1, ss.564-567, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 1
  • Basım Tarihi: 2013
  • Dergi Adı: JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.564-567
  • Anahtar Kelimeler: Orchardgrass, MS medium, 2,4,5-T., tissue culture, plant regeneration, FESTUCA, GROWTH, LOLIUM, VARIABILITY, CULTURE, EMBRYO, TOXIN, 2,4-D, LEAF
  • Çukurova Üniversitesi Adresli: Evet

Özet

This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l(-1) of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg l(-1) of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies.