The present study aimed to investigate phenolic acid composition, antioxidant activity and hepatoprotective potential of Stachys pumila extract (SPE) against CCl4-induced acute hepatotoxicity. Pretreatment of rats with SPE significantly (P<0.01) ameliorated both the increased levels of ALT, AST, OSI, MDA and the decreased activities of SOD and CAT, as compared to CCl4-control group. Histological and biochemical examinations revealed that SPE was more potent than reference drug silymarin in protecting the liver from toxic injury of CCl4 and preserving the hepatocyte ultrastructure. The phenolic acid profile of the methanol extract was determined quantitatively by HPLC/DAD. Ferulic acid was found as the predominant phenolic acid among the analyzed phenolic acids. SPE exerted high antioxidant effect according to in vitro antioxidant activity tests. Taken together, these results suggest that SPE could be a candidate as promising therapeutic agent for treating oxidative stress-related hepatic injury and other diseases.