The Role of Selected Lactic Acid Bacteria on Organic Acid Accumulation during Wet and Spray-Dried Fish-Based Silages. Contributions to the Winning Combination of Microbial Food Safety and Environmental Sustainability

KÜLEY E., ÖZYURT G., ÖZOĞUL İ., BOĞA M., Akyol I., Rocha J. M., ...More

MICROORGANISMS, vol.8, no.2, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.3390/microorganisms8020172
  • Journal Name: MICROORGANISMS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: fish silage, fish residues or by-products, fermentation, lactic acid bacteria, organic acids, lactic acid, acetic acid, formic acid, succinic acid, propionic acid, microbiota and microbial safety, microbial starter cultures, food safety, food preservation, recovery of by-products, environmental sustainability, ESCHERICHIA-COLI, LACTOBACILLUS-PLANTARUM, NUTRIENT DIGESTIBILITY, NUTRITIVE-VALUE, FERMENTATION, PERFORMANCE, GROWTH, INOCULATION, MICROFLORA, SURVIVAL
  • Çukurova University Affiliated: Yes


Organic acid contents of acidified and fermented fish silages made from gibel carp (Caracius gibelio) and klunzinger's ponyfish (Equulites klunzingeri) fishes, and from fish processing residues or by-products, were determined and studied. The silages were undertaken in wet and spray-dried fish-based raw-materials for 3 weeks at room temperature (ca. 25 degrees C). Selected lactic acid bacteria (LAB) of Enterococcus gallinarum, Lactobacillus brevis, Lactobacillus plantarum, Pediococcus acidilactici, and Streptococcus spp. were employed to produce fermented fish-based silages, while acidified silage was prepared resorting to the addition of formic acid (3%, v/v). Lactic and propionic acids were the dominant produced organic acids, while succinic acid was formed at the smallest amounts in fermented silages. In the acidified silage, lactic and formic acids were produced in amounts higher than 800 and 1000 mg (organic acid)/100 g (samples) respectively. Among the fermented fish-based silages, LAB strains unfolded considerable ability to presumptively produce propionic acid in gibel carp silage (>2370 mg (organic acic)/100 g sample). Spray-dried fermented silages displayed significantly higher organic acid content than wet silages. Propionic acid accumulation was found at the highest levels in gibel carp silage fermented with L. plantarum (6335.40 mg (propionic acic)/100 g sample). This research effort pointed out the good capability of various selected lactic acid bacteria strains to produce significant amounts of organic acids especially lactic, acetic, and propionic acids- during the fermentation of fish-based silages. In terms of food safety and quality, such a production of relatively high amounts of organic acids in wet and spray-dried fish-based silages clearly indicated their suitableness to be used for animal feed.