A comparative study of the effects of different doses (2.5 and 5.0 kGy) of gamma radiation on the formation of 12 biogenic amines and ammonia during the storage of sea bream (Sparus aurata) in ice was carried out. No significant differences in ammonia content were observed among the groups, except for 17 days of storage (p > 0.05). Although 12 biogenic amines were determined by HPLC, histamine, tyramine and 2-phenylethylamine were not detected. The main amines formed in sea bream muscle were trimethylamine, putrescine and cadaverine, respectively. Dopamine, serotonin and spermidine concentration remained below the value of 0.6 mg/100 g and did not change significantly for all groups during storage periods (p > 0.05). However, irradiation led to a minor increase in spermine, agmatine and tryptamine concentrations of sea bream in some storage days. The radiation dose used had similar effects on reducing biogenic amine content, except for agmatine, tryptamine.