Akademik Veri Yönetim Sistemi
Tezin Türü: Doktora
Tezin Yürütüldüğü Kurum: Çukurova Üniversitesi, Sağlık Bilimleri Enstitüsü, Temel Tıp Bilimleri, Türkiye
Tezin Onay Tarihi: 2021
Tezin Dili: Türkçe
Öğrenci: İnayet Nur USLU
Danışman: Ayfer Pazarbaşı
Özet:
Exposure to many biological, physical and chemical agents at
every stage of our daily life causes serious health problems, especially in
pregnant women and newborn infants. Unfortunately, exposure to these agents
starts from the fetal period, as the structural formation of the organism is
realized, which we can call the most important phase of our life. Among these,
one of the most important chemical agents is nicotine. Many recent studies
suggest that exposure to nicotine during fetal development has harmful effects
for both mother and fetus. However, the effects of nicotine at the cellular and
molecular level have not yet been clarified. In this study, it is aimed to
reveal the cellular and molecular changes that nicotine can cause in embryonic
stem cells during the neuronal differentiation process.
In our study, the differentiation
process of mouse embryonic stem cells was carried out in vitro. Cells were
divided into two groups as nicotine-exposed and control groups. Gfap, Nestin, O4,
β-3 Tubulin and Map2 proteins of both cell groups were evaluated by
immunohistochemical staining (IHC). In addition, the expressions of NEUROG2,
RBFOX3, TUBB3, GSK3B and PAX6 genes were analyzed by Real Time PCR analysis.
At the end of the analysis, it was
seen that the gene expressions of the group exposed to nicotine decreased
compared to the control group. In the IHC results, it was determined that the
expressions of Map2, β-3 Tubulin and O4 proteins were decreased in the groups
given nicotine compared to the controls.