Protective effect of naringin in rat model of renal ischemia reperfusion injury


Değer M., Akdoğan N., İzol V., Kaplan H. M., Pazarcı P., Arıdoğan İ. A.

Revista de nefrología, diálisis y transplante, vol.41, no.2, pp.113-118, 2021 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 41 Issue: 2
  • Publication Date: 2021
  • Journal Name: Revista de nefrología, diálisis y transplante
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.113-118
  • Çukurova University Affiliated: Yes

Abstract

Objective

We aimed to research that naringin whether protects from renal ischemia/reperfusion induced renal damage in rats.

Methods

Twenty-four Wistar albino female rats randomly were divided into three groups: 1) control group, in which the rats were only performed right nephrectomy; 2) a second group received right nephrectomy and left kidney ischemia (1 h) and reperfusion (24 h) group ischemia/reperfusion (I/R); 3) a third group received 50 mg/kg naringin orally once a day for two weeks before ischemia/reperfusion(I/R/N). Expression of cyclooxygenase-2 (COX-2), cytosolic phospholipase A2 (cPLA2), inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated x protein (Bax), serum creatinine (Cr), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) were measured by using enzyme-linked immunosorbent assay (ELISA).

Results

Naringintreated rats that performed renal ischemia/reperfusion demonstrated significant decrease in Cr, IL-6 and TNF-α levels when compared to the only renal ischemia/reperfusion performed rats. While renal ischemia/reperfusion caused a decrease of bcl-2 (1.72±0.20 pg/ml) levels, while an increase of COX-2 (11882±642 pg/ml), cPLA2 (2448±139 pg/ml), iNOS (4331±438 IU/ml), cleaved caspase-3 (7.33±0.76 ng/ml) and Bax (2.33±0.44 ng/ml) levels. The treatment of naringin reversed these kidney effects (7.47±60.35 pg/ml; 9299±327 pg/ml; 2001±78 pg/ml; 3112±220 IU/ml; 3.38±0.54 ng/ml; 2.33±0.44 ng/ml, respectively)(p< 0.05).

Conclusion

This study showed that naringin treatment attenuated renal damage induced by ischemia/reperfusion in rats.