Simple Extraction and Rapid HPLC Method for Tocopherol Analysis in Marine and Fresh-water Fish Species

ÖZOĞUL F., ÖZOĞUL Y., Kuley E.

FOOD SCIENCE AND TECHNOLOGY RESEARCH, cilt.17, sa.6, ss.595-598, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 6
  • Basım Tarihi: 2011
  • Dergi Adı: FOOD SCIENCE AND TECHNOLOGY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.595-598
  • Anahtar Kelimeler: fresh water fish, marine fish, tocopherol, HPLC method, extraction procedure, PERFORMANCE LIQUID-CHROMATOGRAPHY, ALPHA-TOCOPHEROL, GAMMA-TOCOPHEROL, VITAMIN-E, OXIDATION, PRODUCTS, OIL
  • Çukurova Üniversitesi Adresli: Evet

Özet

A rapid high-performance liquid chromatographic (HPLC) method with simple extraction procedure for the determination of tocopherols in seafood was developed. A continuous isocratic elution system was used for the analysis with a mixture of acetonitrile and methanol. Tocopherols were identified by comparison of retention times and peak area values with standard of alpha-, beta-, gamma- and delta-tocopherols. The HPLC technique used showed good performance parameters (linearity r(2) = 0.9999, and relative standard deviation (RSD) <= 12.38%). The total recoveries for each tocopherol were 99.7, 99.9, 99.8 and 99.7% for alpha, beta, gamma- and delta, respectively. This method was then applied to freshwater and seawater fish species to detect their tocopherol contents. Significant differences (P < 0.05) were observed in the content of tocopherol among fish species. The highest content was obtained from shrimp (81.3 mg/kg), while the lowest was found for whiting (9.8 mg/kg).