The aim of this study was to determine biotypes of Bemisia tabaci (Gennadius 1889) (Hemiptera:Aleyrodidae) collected from different host plants in 2008 and 2009 growing seasons from Adana (Balcali) by using molecular identification methods. Samples were collected from cucumber, eggplants, cotton and soybean grown in Balcali at different dates. RFLP and SCAR-PCR methods were used to determine B. tabaci biotypes. All samples collected form cotton was determined as Q biotype. B biotype was determined only from four samples of totally collected 18 samples in 2008 while from one sample of totally collected 13 samples in 2009. Q type was determined as dominant in Balcali.