The availability of thermal comfort conditions outdoors is considered one of the necessities of being a livable city. There is a relationship between outdoor thermal comfort and microclimate regulation potential of tree species. This study aims to form a proposal planting design, which may enhance outdoor thermal comfort in a university campus. In this study, thermal comfort has been identified by Predicted Mean Vote (PMV) index and ASHRAE scale and the analysis has been performed with ENVI-met software. The study has been carried out in three stages: a) Evaluation of the campus master plan in terms of thermal comfort, b) Formation of planting design, where six tree species have been used to improve thermal comfort and evaluation of this offer in terms of thermal comfort c) Comparison of the findings obtained in the first two stages and manifestation of information, which may be used in the activities aimed at improving thermal comfort. According to the findings obtained in the study, with a planting design in which tree species are located according to their characteristics, PMV values increase by 0.50 on average in the coldest hours of winter and decrease by 1.75 on average in the hottest hours of summer.