The aim of this study was to investigate the antioxadant effects of Hypericum perforatum (HP) and Nigella sativa (NS) in protecting against biochemical and histopathological changes in rat testis injured by experimental ischemia/reperfusion (I/R). This research study was conducted on 40 adult male Wistar albino rats. Rats were randomly separated into four groups of 10 animals each: the sham (control) group in which only the scrotal area opened and closed without torsion and detorsion (T/D); the I/R group, which was T/D applied but not treated by any agent; the HP+I/R, and NS+I/R groups, HP and NS were given intraperitoneally 30 min before T/D 25 mg/kg groups. In the I/R group with HP+IR and NS+I/R treatment groups, torsion was created by rotating only left testis 720 degrees clockwise for 2 hours. At the end of the 2 hours torsion, the left testis was detorsioned and replaced in the scrotum for 4 hours. Along with the completion of the experiment, the left testis was rapidly removed to measure oxidative stress markers and to examine it histopathologically. In the HP+I/R and NS+I/R groups, the malondialdeyhde (MDA) level was significantly lower than in the I/R group only, and the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalaze (Cat) activities were higher than the I/R group. Also our histopathological results supported these biochemical findings. In conclusion, our results suggest that both HP and NS protect against injury of left testis induced by I/R, however the protective effects of NS were more pronounced than those the HP.