Expression of thermostable alpha-amylase gene from Bacillus stearothermophilus in various Bacillus subtilis strains

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Ozcan B. D., Özcan N.

ANNALS OF MICROBIOLOGY, vol.58, no.2, pp.265-268, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 2
  • Publication Date: 2008
  • Doi Number: 10.1007/bf03175327
  • Journal Name: ANNALS OF MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.265-268
  • Keywords: Bacillus stearothermophilus, thermostable alpha-amylase, Bacillus subtilis, electroporation, BACTERIA, CLONING
  • Çukurova University Affiliated: Yes

Abstract

The recombinant plasmid pC194Amy harbouring 5.25 kbp DNA fragment encoding the thermostable of-amylase gene of Bacillus stearothermophilus was introduced into Bacillus subtilis YB886 and B. subtilis BR151 strains by electroporation. The expressing of the alpha-amylase gene in recombinant strains were confirmed on starch plate, SDS-PAGE and zymogram analysis. In the condition of 100 degrees C and 15 min, Y13886Amy, BR151Amy and ATCC31786 alpha-amylases were maintained up to 80, 67 and 52% of their maximum activity respectively. The specific enzyme activities of YB886Amy, BR151Amy and ATCC 31786 were estimated as 2.59, 2.98 and 2.9 mu M/mg protein/min respectively.