The aim of this study was to determine the spawning season, spawning area, and length at first maturity of Pegusa lascaris in Iskenderun Bay (Northeastern Mediterranean Sea). For this purpose, experimental trawl operations in parallel with ichthyoplankton surveys were performed from September 2009 to October 2010 with monthly intervals. Both the results of ichthyoplankton surveys and gonado-somatic index examinations showed that spawning of Pegusa lascaris took place from the end of autumn till the beginning of summer (8 months). Throughout this period, there was two spawning peaks which were in Autumn and Spring. The maximum spawning occurred in April. The spatial distribution of P. lascaris eggs revealed that the main spawning ground was located in 0-50 m depth contours. Female length at first maturity was observed as 14.5 cm.