Akademik Veri Yönetim Sistemi
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.74, sa.1, ss.5119-5130, 2023 (SCI-Expanded)
In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethid-ium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were obtained. The enzyme activities (pH, temperature, thermostability, etc.) of the wild strainand its two mutant variants (UV mutant (mUV), EtBr mutant (mEB)) producing β-1,4-glucanasewere partially characterized. It was found that the optimum pHs for the mUV, mEB, and the wild strain were 7.0, 6.0 and 9.0, respectively; and the optimum temperatures were 50, 60, and 50 °C, respectively. Finally, the molecular weights of the cellulase enzymes of all the three bacteria were found to be 40 kDa by SDS-PAGE and zymogram analysis.undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethid-ium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were obtained. The enzyme activities (pH, temperature, thermostability, etc.) of the wild strainand its two mutant variants (UV mutant (mUV), EtBr mutant (mEB)) producing β-1,4-glucanasewere partially characterized. It was found that the optimum pHs for the mUV, mEB, and the wild strain were 7.0, 6.0 and 9.0, respectively; and the optimum temperatures were 50, 60, and 50 °C, respectively. Finally, the molecular weights of the cellulase enzymes of all the three bacteria were found to be 40 kDa by SDS-PAGE and zymogram analysis.