Biological and Molecular Detection of Citrus Psorosis Virus in Citrus in the Eastern Mediterrenean Region of Turkey

Kayim M.

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.19, sa.2, ss.259-262, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/bf03263352
  • Dergi Adı: JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.259-262
  • Anahtar Kelimeler: Biological indexing, CPsV, C. clementina, C. reticulata, C. sinensis, RT-PCR
  • Çukurova Üniversitesi Adresli: Evet

Özet

Fifty bud sticks exhibiting vein flecking, vein clearing and oak-leaf pattern symptoms of 'Satsuma' and 'Clementine' mandarins, and 'Washington navel' and local oranges in the field in Mersin, Kozan and Adana provinces were collected and grafted on one-year-old sour orange seedlings to maintain Citrus psorosis virus (CPsV) in indicators. Thirty two indicator plants showed typical symptoms of vein flecking, vein clearing, and vein banding with oak-leaf pattern. Eighteen plants gave mild leaf symptoms. CPsV was detected by RT-PCR from CPsV-infected sources. Leaf samples for RT-PCR were collected from symptomatic field trees. In all, thirty samples from different trees of mandarin and twenty samples from different trees of sweet oranges were collected. A 434 bp specific DNA for the coat protein was amplified from the cDNA of CPsV-infected leaf samples. This specific DNA product was not amplified from healthy leaf samples or asymptomatic leaves collected from CPsV-infected trees.