This study investigated the relationship among socio-demographic variables and humor styles on university students' subjective well-being (SWB). Participants were 376 university students attending different faculties at Cukurova University. Positive and Negative Affect, Satisfaction with Life, Humor Styles Questionnaires, and Socio-Demographic Information Questionnaire were used to collect data. To analyze the data, t-test, correlation, and stepwise regression analyses were employed. Aggregate SWB, Aggressive and self-defeating humor of male students were found to be significantly higher than those of female students. However, negative affect female students were significantly higher than those of male students. No significant difference between male and female students with respect to life satisfaction, positive affect, affiliative and self-enhancing humor was detected. Results of regression analyses indicated that self-enhancing humor, aggressive humor, romantic relationship, gender and economic status predict of aggregate SWB.