Association between Obsessive Compulsive Disorder and Tumor Necrosis Factor Gene 308 G A and 850 C T Polymorphisms in Turkish Children

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Lüleyap H. Ü., Onatoğlu D., Tahiroğlu A., Alptekin D., Yılmaz M., Çetiner S., ...More

Balkan Journal of Medical Genetics, vol.15, no.2, pp.61-66, 2012 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 15 Issue: 2
  • Publication Date: 2012
  • Journal Name: Balkan Journal of Medical Genetics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.61-66
  • Çukurova University Affiliated: Yes


Obsessive compulsive disorder (OCD) is a

neurobio-logical disease characterized with obsessions

and compulsions. Obsessive compulsive disorder

occurs with an autoimmune mechanism after

Group A β hemolytic streptococcus (GABHS) infection.

Tumor necrosis factor (TNF) is an important

cytokine, as well as having an important role in the

apoptosis mechanism of autoimmune diseases. It is

expressed by the TNF-α gene. The aim of this study

was to examine the relationship between the TNF-α

gene promoter region –308 (G>A) and –850 (C>T)

polymor-phisms and OCD. In this study, ages of the

OCD patients and the control group ranged between

4 and 12 years. We studied two patient groups, one

included childhood onset OCD patients (n = 49) and

the control group was composed of healthy children

(n = 58). Patients were diagnosed according to the

Diagnostic and Statistical Manual of Mental Disorder

(DSM-IV) criteria and with Kiddie Schedule for

Affective Disorders and Schizophrenia-Present and

Lifetime (KSAD-S-PL) version. For identifying the

poly-morphisms, polymerase chain reaction (PCR),

restriction fragment length polymorphism (RFLP)

and polyacryl-amide gel electrophoresis (PAGE)

methods were used.

For the –308 polymorphism, 45 of 49 OCD

patients’ results were completed, and for the –850

polymorphism, 47 of 49 OCD patients’ results were

completed. According to our statistical results, there

is a positive relationship between OCD and the –308

polymorphism (p <0.001) but no association between

OCD and the –850 polymorphism (p = 0.053). There

is no positive relationship between antistreptolysin O

(ASO) titers and the –308 polymorphism (p = 0.953)

but there is an important significance between the

–850 polymorphism and ASO (p = 0.010). There is

no positive relationship between gender of patients

and OCD (p = 0.180) and no positive association

between ASO and gender (p = 0.467). According to

our results, we hypothesize that we can propose the

mutant AA genotype for the –308 polymorphism, and

that the mutant CT genotype for the –850 polymorphism

may be used as molecular indicators for OCD.

Keywords: Child, Genetic, Obsessive compulsive

dis-order (OCD), Polymorphism


Obsessive compulsive disorder (OCD), atten