Evaluation of the genotoxic or mutagenic effects of thermal stress on cultured human lymphocytes

İLA H. B., TOPAKTAŞ M., Arslan M., BÜYÜKLEYLA M., İSTİFLİ E. S.

TURKISH JOURNAL OF BIOLOGY, cilt.39, sa.1, ss.98-103, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3906/biy-1404-55
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.98-103
  • Anahtar Kelimeler: Heat shock, mitomycin C, in vitro, sister chromatid exchange, chromosome aberration, micronucleus, GAMMA-H2AX FOCI FORMATION, HEAT-SHOCK, SUBSTITUTED CHROMOSOMES, MAMMALIAN-CELLS, ABERRATIONS, SENSITIVITY, MECHANISM, CHROMATIN
  • Çukurova Üniversitesi Adresli: Evet

Özet

This study was performed to determine the cytogenetic effects of short-term thermal stress in human cultured lymphocytes. Experimental heat shock (39 degrees C) was performed alone or with the addition of mitomycin C (MMC) to determine the synergistic or antagonistic effects of heat shock on genotoxicity induced by MMC. In this study, during culture periods of 72 h, human peripheral blood lymphocytes were exposed to heat shock for specified durations (30, 60, 120, and 240 min) 24 or 48 h before harvest. According to our results, the selected temperatures did not show genotoxic or mutagenic effects. In summary, the heat shock tested did not show any cytogenetic effect on the cultured blood cells and did not cause significant alterations in genotoxicity induced by MMC.