Determination of ethyl glucuronide in human hair by hydrophilic interaction liquid chromatography-tandem mass spectrometry


Yaldiz F., DAĞLIOĞLU N. , HİLAL A. , Keten A., GÜLMEN M. K.

JOURNAL OF FORENSIC AND LEGAL MEDICINE, cilt.20, ss.799-802, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 20 Konu: 7
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.jflm.2013.06.013
  • Dergi Adı: JOURNAL OF FORENSIC AND LEGAL MEDICINE
  • Sayfa Sayıları: ss.799-802

Özet

Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been utilized as a marker for alcohol intake. This study presents development, validation and application of a new hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for the analysis of EtG in human hair samples. The linearity was assessed in the range of 5-2000 pg/mg hair, with a correlation coefficient of >0.99. The method was selective and sensitive, with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.05 pg/mg and 0.18 pg/mg in hair, respectively. Differently from the extraction procedures in the literature, a fast and simple liquid-liquid method was used and highest recoveries and cleanest extracts were obtained.