Akademik Veri Yönetim Sistemi
Plant Cell, Tissue and Organ Culture, cilt.163, sa.2, 2025 (SCI-Expanded, Scopus)
In this study, the effects of different nutrient medium replacement intervals (14 days, 21 days, and 42 days [control]) on plant growth parameters and changes in the nutrient medium were evaluated by utilizing the nutrient refreshment advantage of the SETIS™. Critical chemical parameters such as pH changes and the accumulation of phenolic compounds in the nutrient medium were also addressed in this study. During the micropropagation phase, the highest values for plant height, fresh weight, dry weight, photosynthetic pigment content, and stomatal density were recorded in the groups where medium renewal was applied. Similarly, in the rooting phase, significant increases in plant height, biomass accumulation, and stomatal density were observed. A sharp decline in pH values (ranging from 3.12 to 3.84) was detected across all treatments. Compared to the control group (42 days), medium replacement applications provided an advantage in reducing phenolic compound accumulation. The amount of nutrient medium consumed during each replacement interval was measured, revealing that plants grown in refreshed media utilized more medium, which correlated with improved growth parameters. In the acclimatization phase, plantlets derived from the 14-day and 21-day medium replacement groups exhibited stronger and healthier growth. These findings demonstrate that the frequency of nutrient medium replacement has a significant impact not only on the development of banana plants but also on maintaining chemical balance within the culture medium.