Antibiotic resistance is a common and growing threat to public health around the World. Nosocomial infections are one of the most annoying incidences which are causing complications in treatment. Evaluation and surveillance of antibiotic use and resistance increase to pathogens could be great helpfull. This study was aimed to isolate, identify infections agents, Escherichia coli, and determined the prevalance of antibiotic resistance. It was also determined antibacterial activity effect of BAM (Bioactive Molecule).