Bisphenol A decreases expression of Insulin-like factor 3 and induces histopathological changes in the Testes of Rats.

Sencar L., Coskun G., Şaker D., Sapmaz T., Tuli A., Özgür H., ...Daha Fazla

Toxicology and industrial health, cilt.37, ss.314-327, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1177/07482337211014097
  • Dergi Adı: Toxicology and industrial health
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Communication Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, Index Islamicus, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.314-327
  • Anahtar Kelimeler: Testis, bisphenol A, INSL3, testosterone, microscopy, ENDOCRINE DISRUPTOR, FETAL EXPOSURE, INSL3, METABOLISM, TOXICITY, HORMONES, SYSTEM, RXFP2, MICE, BPA
  • Çukurova Üniversitesi Adresli: Evet

Özet

Bisphenol A (BPA) is a chemical agent known to have detrimental reproductive and developmental effects. The tissue-specific impacts of BPA exposures and target tissues sensitiveness to BPA are still unclear. The aim of this study was to determine the short- and long-term dose-dependent toxic effects of BPA on rat testes. Forty-eight Wistar albino male rats were divided into four groups each containing 12 rats. To induce toxicity, BPA was administered orally at three different dosages (50, 100, and 200 mg/kg) for 14 and 28 days, respectively. Testis tissues were examined using light and electron microscopy, immunohistochemistry, and biochemical methods. Serum testosterone (T) and luteinizing hormone (LH) levels were measured. Additionally, insulin-like factor 3 (INSL3) as a marker of Leydig cell function was evaluated immunohistochemically. Groups administered high doses of BPA showed severe degenerations such as testicular atrophy, spermatogenic arrest, and interstitial edema in testis. Also, a significant decrease in INSL3 immunoreactivity and serum LH and T levels was found. The results indicated that both increased exposure time and dosage of BPA caused more serious detrimental effects on testes in the rat. Decreased INSL3 and T levels was evidence of Leydig cell function impairment due to BPA.