Tricholoma anatolicum collected from Turkey is consumed by public and exported to Japan every year. It was previously identified as Tricholoma caligatum until it was recognized as a new species. In the existing literature, there is no information on the aromatic composition of T. anatolicum. Therefore, in this study it was aimed to identify the volatile composition of T. anatolicum together with T. caligatum. Species identification was confirmed using molecular analyses based on ITS rDNA sequencing. Volatile compounds of both mushroom species were extracted using liquid-liquid extraction method and determined by Gas Chromatography-Mass Spectrometry-Flame Ionization Detector (GC-MS-FID). In the two Tricholoma species, 31 volatiles were obtained and grouped in seven chemical classes. The amounts of alcohols, volatile acids and esters were found to be higher in T. anatolicum, whereas the amounts of terpenes were detected as higher in T. caligatum. 1-Octen-3-ol responsible for the mushroom-like odour was only found in T. anatolicum.