Production and characterization of multifunctional endoxylanase by Bacillus sp X13


Aygan A., ARIKAN B.

TURKISH JOURNAL OF BIOLOGY, cilt.33, ss.231-237, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 33 Konu: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.3906/biy-0806-18
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Sayfa Sayısı: ss.231-237

Özet

A multifunctional endoxylanase producing Bacillus sp. was isolated from soil. Enzyme synthesis occurred at temperatures between 20 degrees C and 60 degrees C with an optimum 37 degrees C. Analysis of the enzyme by SDS-PAGE revealed 4 active enzyme bands, which were estimated to be 66.5 kDa, 80.6 kDa, 95.5 kDa, and 108.4 kDa. The enzyme has a broad temperature range, between 20 and 90 degrees C, with an optimum at 40 degrees C; and maximum activity was at pH 6.0. The enzyme showed a gradual decrease in the remaining activity as the pre-incubation temperature increase. Thermostability was not also increased in the presence of Ca2+. An average of 71% of remaining activity observed when the enzyme incubated between pH 3.6 and 10 for 1 h. The enzyme was highly inhibited by urea, and fairly inhibited by Triton X-100, CaCl2, ZnCl2, KCl, Na2SO3 PMSF, and 2-Mercaptoethanol. The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest.