Akademik Veri Yönetim Sistemi
Plant Biosystems, 2025 (SCI-Expanded)
Apple is one of the most consumed fruits in the world and has many local varieties. This study aims to determine the bioactive compounds and properties of the Amasya apple, the most widely cultivated local apple variety in Turkey. For this purpose, phytochemicals were extracted from the peel and flesh of the apple using ultrasonic extraction with ethanol, methanol, water, and hexane solvents. The total phenolic (TPC) and flavonoid (TFC) contents of the extracts were determined, and the phenolic compounds were analyzed using LC/MS-MS. Additionally, the antioxidant capacities (DPPH and FRAP), enzyme inhibition activities (α-amylase, α-glucosidase, and acetylcholinesterase (AChE)), and antibacterial activities of the extracts were evaluated. The most abundant phenolic compound was epicatechin, followed by catechin. The inhibitory effect of water (9.77 mg/L) and methanol (12.37 mg/L) peel extracts on α-amylase was higher than the positive control (acarbose, 13.34 mg/L). Only the peel extracts inhibited AChE. The antibacterial activities of the extracts against Escherichia coli ATCC 25922 (E. coli) and Staphylococcus aureus ATCC 29213 (S. aureus) varied according to the solvents used. According to the results of this study, the peel parts of the apple and the water extracts contain more bioactive compounds and exhibit better-related properties.