RAPID TEST KITS USED IN DETERMINING OF ANTIBIOTIC RESIDUES IN MILK AND THE RELIABILITY OF KITS


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Özdemir Kütahya Z. , Traş B.

4TH INTERNATIONAL CONFERENCE AND INDUSTRIAL EXHIBITION ON DAIRY SCIENCE PARK, Konya, Turkey, 1 - 05 November 2017, pp.269

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.269

Abstract

Antibacterial drugs are the most commonly used group of veterinary drugs. In lactation cows, antibiotics are used in the treatment of various infectious diseases such as mastitis. Antibacterial drugs pass into milk at different levels. This situation can cause health problems in humans and economic loss in food manufacturing. The screening methods used in the determination of antibacterial residues in animal foods are bioanalytical and semi-quantitative methods. These methods, which could be performed in in-situ environments, could be classified as microbiological, immunochemical and biosensor methods. There are a lot of commercial kits for screening residue in the milk. The advantages of commercial test kits are that they give quick results and easy application in the field. The disadvantages are that they are non-specific, give false positive/negative results and cause economic loss due to the positive results of residues below safety limits. The reasons of false-positive results in commercial test kits are natural antibacterial substances in colostrum and mastitis milk, the presence of high fat and protein concentration, detergent and disinfectant residues, storage conditions of milk samples and the presence of antiparasitic drug residues in milk. Different sensitivities and results can be observed with distinct commercial test kits used for the same samples. The reasons of this difference can be the working principle of the test kits and the difference in species. Therefore, commercial kits may not always yield reliable results. Chromatographic methods are the most appropriate method for identifying and validating of residues. Antibacterials and other drugs in animal foods have been scanned for the last 11 years by the "Residual Monitoring Program" in Turkey. Authorized laboratories continue to residue analyses of milk samples taken at regular intervals. Residues of drugs in food are more strictly monitored by authorized institutions for public health.