Pesticides are used to protect crops and to eliminate pests, though non-target organisms such as mammals are also affected from their usage. Etoxazole (organoflourine pesticide) is an acaricide used to combat spider mites which are the parasites of various crops. The present study aims to investigate the effects of etoxazole on the level of MDA (malondialdehyde) and activities of CAT (catalase), GPx (glutathione peroxidase), and AChE (acetylcholinesterase) in liver and kidney tissues of Wistar rats (Rattus norvegicus var. albinos). Rats received etoxazole intraperitoneally with doses of 2.2, 11, and 22 mg/kg b.w./day for 21 days. Control rats received the same volume of the serum physiologic. Following etoxazole exposures, activities of CAT, GPx, and AChE in the liver and kidney of rats significantly decreased at all doses compared to control group. Oppositely, MDA levels in these tissues increased significantly at all doses following etoxazole exposures. The present study demonstrated that etoxazole, at all doses, had toxic effects in the liver and kidney parameters, suggesting their possible use as effective biomarkers in determining the toxic effects of etoxazole. This may suggest that these biomarkers could also be used as a tool to monitor pesticide-affected areas before severe toxic effects begin in non-target animals and humans.