In this study, using of nettle fiber, a natural fiber, in towel production and the selected performance properties were examined. Six different towel samples were produced in the study. Ground warp yarns of all towels are cotton. Three different raw materials as weft yarns and two different raw materials as pile warp yarns were selected. The yarn containing nettle fiber (70% cotton/30% nettle) was used in weft direction. The performance parameters such as water absorbency, softness, quick drying of samples, and antibacterial activity for two bacteria were investigated for before and after household washing. As a result, it was seen that the nettle fiber can be used as an alternative in towel industry.