In this study, the effects of the teaching of the life science course performed using Storyjumper software- one of the Web 2.0 tools-, on primary school second grade students' cultural awareness and creative thinking skills were examined. In the study, single group pretest-posttest weak experimental model was used. Convenience sampling method was used for determining the participants of the research. The participants of the research consisted of 22 second grade students. In order to collect the research data, Torrance Test of Creative Thinking Verbal-A and Cultural Awareness Level Determination Form created by the researchers were used. The analysis of quantitative data was conducted via Wilcoxon signed-ranks test. The answers for Cultural Awareness Level Determination Form were analyzed using inductive content analysis methods. As a result of the study, it was determined that the teaching performed for digital story creation had positive effects on students' cultural awareness and creative thinking skills.