2,2-bis(3,3,4,7-tetra hydroxyflavone) 1,3-dicyclohexyl carbamine, 3,3,4,7-tetra hydroxyflavone was obtained by the reaction of 3,3,4,7-tetra hydroxyflavone with 1,3-dicyclohexyl carbodiimid in presence of 4-dimethyl amino pyridine. The above obtained ligand can chelate with trivalent metal ion. Hematologic and pathologic data of the dinuclear iron complex with tetrahedral geometry were discussed. The ability of the synthesized ligand as a chelator to remove overloaded iron from rat liver and spleen tissues was studied. The results show that the chelate effect of the ligand as the bidentate is near that of desferrioxamine available in market in oral applications for the treatment of beta-thalassemia.