Non-renewable phosphorus (P) resources are intensively declining and recyclable P is high in demand for agricultural sector. Biochar as a renewable source of P and its physicochemical properties may improve the nutrients condition in the soil for plant availability. This study was designed to evaluate the interaction of biochar with soil microbes in differently textured and P-limited soils for P availability, root colonization and nutrient uptake by plants. Onion plants were grown in two differently textured soils with two types of biochar, with or without P application, three microbially inoculated treatments and uninoculated control. Plants were grown for 65 days and root-shoot biomass, nutrient concentration and mycorrhizal root colonization were analyzed. The WinRhizo was used to evaluate root attributes such as length, surface area and volume of roots. Biochar addition enhanced the nutrient uptake and plant biomass in the presence of P and microbial inoculants. Root colonization was notably increased in biochar + mycorrhizal inoculated plants. Biochar and soil type interactions may develop a unique behavior of nutrient uptake, root colonization, plant growth and root attributes. Biochar in combination with microbial inoculants could be considered a potentially renewable source of P fertilizer.