Determination of milk/plasma rate and, the milk and plasma pharmacokinetics of amoxicillin in dairy cattle


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Özdemir Kütahya Z. , Traş B., Üney K., Eser H., Beşoluk T. M.

7th International Molecular Biology and Biotechnology Congress, Konya, Turkey, 25 - 27 April 2018, pp.98

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.98

Abstract

The present study was conducted to determine the passage ratio of amoxicillin into milk and, pharmacokinetics of amoxicillin in milk and plasma after intramuscular administration. In this study, a total of 5 healthy dairy cattle (Holstein, 450- 500 kg, 2-4 years) were used. Animals received a single intramuscular amoxicillin trihydrate at a dose of 14 mg/kg bw. Blood samples were collected from the jugular vein into tubes with EDTA prior to drug administration (0) and at 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 h after administration. Milk samples were collected prior to antibiotic administration (0) and at 0.25, 0.5 0.75, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 h after administration. The plasma and milk concentrations of amoxicillin were determined using HPLC system with UV detector. The passage ratio of amoxicillin into milk was determined by both AUC-based calculation and using milk and plasma concentrations at sampling times .The milk/plasma ratio of amoxicillin was found 0.46-052. The terminal half-life and MRT parameters of amoxicillin in plasma and milk were determined 6.05 and 2.62 and 8.60 and 5.35 h, respectively. The Cmax levels of amoxicillin in plasma and milk were measured as 1096 and 457.25 ng/mL, respectively. In conclusion, it may be stated that amoxicillin exhibits similar pharmacokinetic behavior profile in plasma and milk.