The present experiment was carried out during 2011 - 2013 open-field cultivation in order to determine the effects of black plastic, agro-textile and sawdust mulches on ascorbic acid (AA), total phenolic content (TPC) and antioxidant activity (DPPH test) of organically grown strawberries (Fragaria x ananassa Duch.) cv. Monterey, Albion, Aromas, Camarosa and Sweet Charlie. TPC was determined by Folin - Ciocalteu (FCR) method, while antioxidant activity was determined by DPPH radical scavenger. The AA, TPC and DPPH of strawberry fruits were significantly influenced by genotypes difference and mulch types. Camarosa cultivar was found superior in terms of AA (61.49 mg/100 gr), TPC content (2140.45 mg GAE/kg w) and DPPH radical scavenger (3.76 1.1 g/mL). In addition, sawdust mulch had a higher impact on TPC (1820.60 mg GAE/kg fw) and DPPH radical (4.56 mu g/mL) while agro-textile mulch had a higher impact on AA content (58 mg/100 gr).