In this study, 8 hearts of adult donkeys of both sexes were examined grossly. In the right ventricle, Musculus papillaris magnus (mp magnus) was observed to have 2-4 processing nipple like processes, which gave rise to Chordae tendineae (CT) that were observed to divide into 7-12 subbranches. Musculi papillares parvi (mmp parvi) were determined to have 2-3 headed nipple-like structures. The total number of CT varied between 7-12. Musculus papillaris subarteriosus (mp subarteriosus) had either no process or 1-3 process (headed nipple). The number of CT arising from this muscle was observed to vary between 8-11 in the left ventricle. The number of CT that originated from Musculus papillaris subatrialis (mp subatrialis) and Musculus papillaris subauricularis were determined to be 4-12 and 6-14, respectively. All of the CT divided, in turn, into subbranches before attaching to the apex, body and base of the cusps. Trabecula septomarginalis (TS) were observed to exist in both the right and left ventricles in all of the examined animals.