Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes

EVECEN M., Cirit Ü., Demir K., Ozdas O. B., Tas M., Birler S., ...Daha Fazla

ANIMAL REPRODUCTION SCIENCE, cilt.117, ss.160-165, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 117
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.anireprosci.2009.03.004
  • Dergi Adı: ANIMAL REPRODUCTION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.160-165
  • Çukurova Üniversitesi Adresli: Hayır

Özet

Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The present study investigated the effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. The donor bitches were categorized into three groups based on stage of estrus cycle: follicular (proestrus or estrous), luteal (diestrus) and anestrus. One ovary of each pair collected from 39 mature bitches was transported in Phosphate Buffer Saline (PBS) at 4 degrees C while the other was transported at 37 degrees C. A total of 1138 Grade I COCs obtained from all ovaries were grouped and matured in modified synthetic oviduct fluid (mSOF) supplemented with follicle stimulating hormone (FSH), luteinizing hormone (LH), essential and non-essential amino acids at 38.5 degrees C in a humidified 5% CO2, 5% O-2, and 90% N-2 atmosphere for 72 h. The nuclear maturation rates were evaluated by aceto-orcein staining.