IDENTIFICATION AND GENETIC DIVERSITY OF MELOIDOGYNE CHITWOODI IN POTATO PRODUCTION AREAS OF TURKEY

Devran Z., Mutlu N., ÖZARSLANDAN A., ELEKCİOĞLU İ. H.

NEMATROPICA, cilt.39, sa.1, ss.75-83, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 1
  • Basım Tarihi: 2009
  • Dergi Adı: NEMATROPICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.75-83
  • Çukurova Üniversitesi Adresli: Evet

Özet

Root-knot nematodes (Meloidogyne spp.) were first detected in Turkey during regional nematode surveys of potato fields of Nigde province. Twelve populations of Meloidogyne were collected and identified based on morphological characteristics as well as molecular methods including species-specific primers, confirming that all twelve populations were M. chitwoodi. To estimate genetic relationships among these twelve populations, random amplified polymorphic DNA (RAPD) analysis was done and the results were subjected to UPGM analysis. Analysis of 394 RAPD markers resulted in a similarity coefficient, ranging between 0.64 and 0.81 with a mean similarity of 0.72. The populations Mc3 and Mc4 displayed the greatest genetic similarity (81%). The lowest level of similarity (65%) was observed between Mc9 and Mc7. The large genetic variation among the M. chitwoodi populations indicates that multiple sources of inoculum might have been introduced to the region or that the populations formerly existed in these potato growing areas of Turkey.

Devran, Z., M. Nedim, Ö. Adem, and E.

.

I. Halil.

2009. Identification and genetic diversity of

Meloidogyne

chitwoodi

in potato production areas of Turkey. Nematropica 39:75-83.

Root-knot nematodes (

Meloidogyne

spp.) were first detected in Turkey during regional nematode

surveys of potato fields of Ni?gde province. Twelve populations of

Meloidogyne

were collected

and identified based on morphological characteristics as well as molecular methods including

species-specific primers, confirming that all twelve populations were

M. chitwoodi

. To estimate genetic

relationships among these twelve populations, random amplified polymorphic DNA

(RAPD) analysis was done and the results were subjected to UPGM analysis. Analysis of 394 RAPD

markers resulted in a similarity coefficient, ranging between 0.64 and 0.81 with a mean similarity

of 0.72. The populations Mc3 and Mc4 displayed the greatest genetic similarity (81%). The lowest

level of similarity (65%) was observed between Mc9 and Mc7. The large genetic variation

among the

M. chitwoodi

populations indicates that multiple sources of inoculum might have been

introduced to the region or that the populations formerly existed in these potato growing areas

of Turkey.

Key words:

Columbia root-knot nematode, genetic similarity, identification,

Meloidogyne chitwoodi

, PCR,

RAPD.