Recent advances in the modern techniques of propagation could help growers to meet the demand of the ornamental industry. New ornamental plant varieties are being created by breeders in response to consumer demand for new products. In this study, different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l) and IAA (0.0, 0.5, 1.0 mg/l) were investigated to optimize regeneration of Gazania rigens. The best callus formation was found from the media containing 0.2 mg/l BA-1.0 mg/l IAA (100%), 0.5 mg/l BA-1.0 mg/l IAA (98%), 0.5 mg/l BA-0.5 mg/l IAA (97%), 1 mg/l BA -1 mg/l IAA (97%), 1.0 mg/l BA-0.5 mg/l IAA (98%). 0.5 mg/l BA (97%). The most promising regeneration and growth were obtained from the media with 0.2 mg/l BA-1.0 mg/l IAA (30%). 1.0 mg/l BA-0.5 mg/l IAA (22%) and 1.0 mg/l BA (18%). The regenerated plantlets were rooted on the media containing 0.5 and 1.0 mg/l IAA. The best rooting percentage was observed on the media containing 1.0 mg/l IAA (75%).