Development of an efficient in vitro regeneration protocol for temperate mulberry (Morus alba L. var. Ichinose) through callus-mediated organogenesis
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42535-026-01946-7
- Dergi Adı: Vegetos
- Derginin Tarandığı İndeksler: Scopus, Chemical Abstracts Core, Natural Science Collection (ProQuest)
- Anahtar Kelimeler: 2, 4-D, callus, Ichinose, In vitro regeneration, Morus albaL
- Çukurova Üniversitesi Adresli: Evet
Özet
Temperate mulberry (Morus alba L. var. Ichinose) is an important plant species for sericulture, but its large-scale propagation is limited by the lack of an efficient and reproducible in-vitro regeneration protocol through the callus phase. The objective of this study was to develop an efficient in-vitro regeneration protocol for temperate mulberry through the callus phase. Callus cultures were raised from explants of Morus alba L. var. Ichinose on Murashige and Skoogs medium supplemented with different plant growth regulators. Full sterilization of nodal explants was obtained on 0.1% HgCl2 for 8 min with 80% survival rate. The leaf explants demonstrated 70% survival rate and a total sterilization on 0.1% HgCl2 for 6 min. The maximum response for callus development with White and Friable Callus was obtained from leaf explants on MS + NAA (2.00 µM) with 93.53% callusing percentage followed by Light Brown and Friable Callus from nodal explants on MS + 2,4-D + NAA (1.00 + 2.00 µM) with 92.16% callusing percentage. On MS media supplemented with different concentrations of growth regulators (IAA/IBA/IAA + IBA), root development was reported. But IBA was shown to be more effective for micro-shoot rhizogenesis. Hardening was achieved with 70% and 60% survival rate from leaf callus and nodal callus regenerated plants respectively. An efficient and reproducible indirect regeneration protocol for Morus alba L. var. Ichinose was successfully established through the callus phase. The optimized culture conditions support rapid clonal propagation, production of healthy planting material, and provide a reliable platform for future genetic improvement, conservation, and biotechnological applications in temperate mulberry.