Catalase and Superoxide Dismutase Enzymes Activities in Flaxseed (Linum Usitatissimum) Extract

YÜCEBİLGİÇ G. , Bozdemir Y.

ASIAN JOURNAL OF CHEMISTRY, vol.22, no.3, pp.2013-2017, 2010 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 3
  • Publication Date: 2010
  • Title of Journal : ASIAN JOURNAL OF CHEMISTRY
  • Page Numbers: pp.2013-2017

Abstract

In this study, catalase (E.C. 1.11.1.6) and superoxide dismutase (EC 1.15.1.1) activities were determined in the extract of flaxseed (Linum usitatissimum) with high antioxidant effect. Activities were determined after homogenization, ultracentrifugation and PD-10 (Sephadex G-25M) column chromotography steps. After the ultracentrifugation step, catalase and superoxide dismutase specific activities were found as 15.94 U/mg prot. and 2.05 U/mg prot., respectively. After desalting with PD-10 column chromotography, catalase and superoxide dismutase specific activities were found as 17.56 U/mg prot. and 4.90 U/mg prot., respectively.