Catalase and Superoxide Dismutase Enzymes Activities in Flaxseed (Linum Usitatissimum) Extract
ASIAN JOURNAL OF CHEMISTRY, cilt.22, sa.3, ss.2013-2017, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 22 Sayı: 3
- Basım Tarihi: 2010
- Dergi Adı: ASIAN JOURNAL OF CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2013-2017
- Çukurova Üniversitesi Adresli: Evet
Özet
In this study, catalase (E.C. 1.11.1.6) and superoxide dismutase (EC 1.15.1.1) activities were determined in the extract of flaxseed (Linum usitatissimum) with high antioxidant effect. Activities were determined after homogenization, ultracentrifugation and PD-10 (Sephadex G-25M) column chromotography steps. After the ultracentrifugation step, catalase and superoxide dismutase specific activities were found as 15.94 U/mg prot. and 2.05 U/mg prot., respectively. After desalting with PD-10 column chromotography, catalase and superoxide dismutase specific activities were found as 17.56 U/mg prot. and 4.90 U/mg prot., respectively.