Comparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen

Cirit Ü., Bagis H., Demir K., Agca C., PABUCCUOĞLU S., Varisli O., ...Daha Fazla

ANIMAL REPRODUCTION SCIENCE, cilt.139, ss.38-44, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 139
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.anireprosci.2013.03.010
  • Dergi Adı: ANIMAL REPRODUCTION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.38-44
  • Çukurova Üniversitesi Adresli: Hayır

Özet

This study was conducted to improve cryosurvival of electroejaculated (EE) ram semen in the presence of iodixanol (OptiPrep (TM)), trehalose or cysteamine. A tris-based extender was used to prepare 12 extenders containing OptiPrep (TM) (Op), trehalose (Tr) or cysteamine (Cy) alone, or different combinations of these compounds. Extenders were designated as follows: Tris (control), Op1.25 (1.25% Op, v/v), Op2.5 (2.5% Op, v/v), Op5 (5% Op, v/v), Tr50 (50 mM Tr), Tr100 (100 mM Tr), Cy (5 mM Cy), OpTr (2.5% Op and 100 mM Tr), OpCy (2.5% Op and 5 mM Cy), TrCy (100 mM Tr and 5 mM Cy), OpTrCy1 (2.5% Op, 100 mM Tr and 5 mM Cy) and OpTrCy2 (1.25% Op, 50 mM Tr and 2.5 mM Cy).