Aim of the study was to describe an in vitro method for evaluation of iron chlorosis responses of grapevine (Vitis sp.) genotypes. Nodal green segments of three rootstocks, 'Fercal', '1103 V, '1613 C' and one table grape cultivar, 'Perlette' were cultured under in vitro conditions. Three iron concentrations (9, 18, 36 mg L-1 FeNaEDTA) alone and their combining effects with 840 mg L-1 NaHCO3 were compared in the MS medium supplemented with 4.4 mu M BA. Grapevine genotypes were examined for leaf chlorosis rating scales, total chlorophyll content; shoot dry weights of the plantlets, and total and active Fe concentrations of leaves.