The Ispendere ophiolite forms part of the Tauride active continental margin assemblage in SE Anatolia. The ophiolite exhibits an intact oceanic lithosphere section and is intruded by Late Cretaceous calc-alkaline granites. The ophiolite comprises mantle tectonites, ultramafic to mafic cumulates, isotropic gabbros, isolated diabase dykes, a sheeted dyke complex, plagiogranite and volcanic rocks. The volcanics and the sheeted dyke complex exhibit (1) similar rare earth element patterns, with flat to light rare earth element depletion (La-Yb)(N) = 0.71-1.14 and 0.65-1.22, (2) negative Nb anomalies and (3) flat-lying high field strength element trends. These features differ from a typical Normal-Mid Ocean Ridge Basalt fractionation trend and could have resulted from c. 15% partial melting of a previously depleted mantle source. The whole-rock chemistry and the mineral chemistry of the ultramafic to mafic cumulates [high Ca plagioclases (An(89-81)), magnesian olivines (Fo(88-81)) and clinopyroxenes (Mg#(90-83))] show that the primary magma of the plutonic suite is compositionally similar to modern island arc tholeiites. The available evidence suggests that the Ispendere ophiolite formed at a northerly supra-subduction zone spreading centre of the Southern Neotethys, between the Taurides and the Bitlis-Puturge metamorphic units, during the Late Cretaceous. Comparison with the adjacent Goksun, Komurhan and Guleman ophiolites suggests that the Ispendere ophiolite represents part of a single regional-scale sheet of oceanic lithosphere that was accreted to the base of Tauride active continental margin where it was cut by arc-type magmatic rocks.