POTENTIAL OF BACTERIOPHAGES TO CONTROL BACTERIAL SPECK OF TOMATO (PSEUDOMONAS SYRINGAE pv. TOMATO)


Cement A., Saygili H., HORUZ S., AYSAN Y.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.27, ss.9366-9373, 2018 (SCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 27
  • Basım Tarihi: 2018
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Sayfa Sayıları: ss.9366-9373

Özet

Tomato (Solanum lycopersicum L.), is one of the widely grown vegetable crop throught the world. The bacterium, Pseudomonas syringae pv. tomato (Pst), causes bacterial speck disease on tomatoes. Several control strategies are performed for disease management. As a strategy, bacteriophages, are natural enemies of bacteria and extremely specific to their targeted hosts without affecting any other bacteria. In this study, isolation of Pseudomonas syringae pv. tomato-lysing bacteriophages, potential of bacteriophages as biological control agents and efficacy of phages on other tomato infecting and saprophytic bacteria were investigated. Samples of tomato plants with typical symptoms of bacterial speck disease were collected from 17 tomato fields, 12 greenhouses and four nurseries in Adana and Mersin provinces in the Eastern Mediterranean Region of Turkey. The plaques (inhibition zones) were observed after 36 hours of incubation, and 47 putative bacteriophages were purified. Among the obtained bacteriophages, phage PH 33 isolate was completely (100 %) suppressed the growth of pathogen and increased the germination rate by %14.6, whereas the phage PH 34 was suppressed the pathogen by 65.8 % and increased the germination rate by 21.6 %. These phages (PH 33 and PH 34) had no inhibition effect on the other tomato bacterial diseases and the saprophytic bacterial flora on tomatoes. Both bacteriophages were specific to Pseudomonas syringae pv. tomato. To our knowledge, this is the first detailed study about isolation of bacteriophages against Pst and efficacy of bacteriophages for biocontrol of Pst in Turkey.