Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes


SERIN M. B. , WAKI K., CHANG K., ASLAN G., DIREKEL S., OTAG F., ...More

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.57, no.3, pp.295-299, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.diagmicrobio.2006.09.001
  • Title of Journal : DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
  • Page Numbers: pp.295-299

Abstract

Forty-six independent isolates of Leishmania subgenus were examined by restriction fragment length polymorphism (RFLP) analyses of polymerase chain reaction (PCR)-amplified mimexon sequences. The results were consistent with grouping of these isolates into species complexes based on sequence polymorphism of single-copy genes. Mimexon RFLP profiles specific to Leishmania enriettii, Leishmania tarentolae, and Leishmania gerbilli were obtained for the first time. The mimexon PCR-RFLP method is thus further confirmed as a simple, sensitive, and specific method useful for discriminating Leishmania isolates. (c) 2007 Elsevier Inc. All rights reserved.