Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes

SERIN, MEDİHA; WAKI, Kayoko; CHANG, Kwang-Poo; ASLAN, Gonul; DIREKEL, Sahin; OTAG, Feza; Kayar, Begum; Koksal, FATİH; EMEKDAS, Gurol

doi:10.1016/j.diagmicrobio.2006.09.001

Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes

SERIN M. B., WAKI K., CHANG K., ASLAN G., DIREKEL S., OTAG F., ...Daha Fazla

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, cilt.57, sa.3, ss.295-299, 2007 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 57 Sayı: 3
Basım Tarihi: 2007
Doi Numarası: 10.1016/j.diagmicrobio.2006.09.001
Dergi Adı: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.295-299
Çukurova Üniversitesi Adresli: Evet

Özet

Forty-six independent isolates of Leishmania subgenus were examined by restriction fragment length polymorphism (RFLP) analyses of polymerase chain reaction (PCR)-amplified mimexon sequences. The results were consistent with grouping of these isolates into species complexes based on sequence polymorphism of single-copy genes. Mimexon RFLP profiles specific to Leishmania enriettii, Leishmania tarentolae, and Leishmania gerbilli were obtained for the first time. The mimexon PCR-RFLP method is thus further confirmed as a simple, sensitive, and specific method useful for discriminating Leishmania isolates. (c) 2007 Elsevier Inc. All rights reserved.