Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes

SERIN, MEDİHA; WAKI, Kayoko; CHANG, Kwang-Poo; ASLAN, Gonul; DIREKEL, Sahin; OTAG, Feza; Kayar, Begum; Koksal, FATİH; EMEKDAS, Gurol

doi:10.1016/j.diagmicrobio.2006.09.001

Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes

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SERIN M. B., WAKI K., CHANG K., ASLAN G., DIREKEL S., OTAG F., ...More

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.57, no.3, pp.295-299, 2007 (SCI-Expanded)

Publication Type: Article / Article
Volume: 57 Issue: 3
Publication Date: 2007
Doi Number: 10.1016/j.diagmicrobio.2006.09.001
Journal Name: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.295-299
Çukurova University Affiliated: Yes

Abstract

Forty-six independent isolates of Leishmania subgenus were examined by restriction fragment length polymorphism (RFLP) analyses of polymerase chain reaction (PCR)-amplified mimexon sequences. The results were consistent with grouping of these isolates into species complexes based on sequence polymorphism of single-copy genes. Mimexon RFLP profiles specific to Leishmania enriettii, Leishmania tarentolae, and Leishmania gerbilli were obtained for the first time. The mimexon PCR-RFLP method is thus further confirmed as a simple, sensitive, and specific method useful for discriminating Leishmania isolates. (c) 2007 Elsevier Inc. All rights reserved.