Apoptotic gene expression profiles and DNA damage levels in rat liver treated with perfluorooctane sulfonate and protective role of curcumin

EKE D., Celik A., YILMAZ M. B., Aras N., Sel S. K., ALPTEKİN D.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.104, pp.515-520, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 104
  • Publication Date: 2017
  • Doi Number: 10.1016/j.ijbiomac.2017.06.075
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.515-520
  • Keywords: Apoptosis, Caspase 3 and 8, Liver cells, Micronucleus, Perfluorooctane sulfonate, Single cell gel electrophoresis, INDUCED GENOTOXICITY, BONE-MARROW, OXIDATIVE STRESS, PFOS, INDUCTION, EXPOSURE, BCL-2
  • Çukurova University Affiliated: Yes


Perfluorinated compounds (PFCS) such as PFOS and PFOA, are xenobiotics that can be detected worldwide in the environment and humans. PFOS (C8F17SO3-) is a fluorinated organic compound has been used for decades in industrial and commercial products. We investigated the genotoxic and apoptotic impact of PFOS in rat liver using comet assay, micronucleus test and apoptotic gene expression methods for caspase 3, caspase 8 and the protective role of curcumin on the PFOS-induced damage under chronic exposure. In this study, rats were treated either with three different PFOS doses only (0.6, 1.25 and 2.5 mg/kg) or one dose of curcumin (80 mg/kg) or three different doses of PFOS combined with 80 mg/kg dose of curcumin by gavage for 30 days at 48 h intervals. We evaluated the DNA damage via comet assay and micronucleus test. Doses of PFOS increased micronucleus frequency (p < 0.05) and strongly induced DNA damage in liver in two different parameters; i: the damaged cell percentage and ii: genetic damage index. Curcumin prevented the formation of DNA damage induced by PFOS and curcumin substance applied with PFOS caused a decrease in the micronucleus frequency. PFOS increased apoptotic gene expression but curcumin decreased the expression levels of caspase 3 and 8. (C) 2017 Elsevier B.V. All rights reserved.