Genetic diversity was evaluated by sequence related amplified polymorphism (SRAP) markers among 27 grapefruit (Citrus paradisi Macf.), 5 pummelo (Citrus maxima (Burm.) Merr.) accessions and 4 pummelo hybrids. Twenty-one SRAP primer combinations produced a total of 90 polymorphic fragments with an average of 4.3 per primer combinations. The mean polymorphism information content (PIC) was 0.34. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity range from 0.73 to 1.00. Accessions were separated into two main clusters and one of them included 5 pummelos whereas the other one included grapefruits. In the pummelo cluster all pummelos were distinguished whereas in the grapefruit cluster most of grapefruits did not clearly separate from each other probably due to their mutation origin.