Akademik Veri Yönetim Sistemi
Journal of Clinical Laboratory Analysis, 2026 (SCI-Expanded, Scopus)
Objective: To investigate immunophenotypic alterations in regulatory T cells (Tregs) and neutrophil dynamics in adult sickle cell anemia (SCA) patients during painful crises and steady state. Methods: Ninety-three participants were included: 17 SCA patients in painful crisis, 27 in steady state, and 49 healthy controls. Flow cytometry was used to assess CD3+CD4+CD25+FoxP3+ Tregs and related subsets. Hematological parameters were evaluated by complete blood counts. Statistical analyses included group comparisons, multiple regression, and ROC curve analysis. Results: SCA patients exhibited significant reductions in CD25+ and CD4+ T cell subsets, despite preserved FoxP3+ Treg frequencies. White blood cell and neutrophil counts were elevated, especially during crisis. Neutrophil and lymphocyte percentages significantly predicted T cell subset levels. ROC analysis identified CD3+ and CD4+ percentages as strong classifiers of disease state. Conclusion: Despite numerical preservation of FoxP3+ Tregs, SCA is marked by impaired T cell activation and sustained innate immune activation. These immune shifts may relate to but do not directly determine end-organ complications. Functional assays and longitudinal studies are needed to elucidate Treg competence, hydroxyurea effects, and age-related immune changes in SCA.