Beneficial Effects of Rosuvastatin and L-Arginine on High Glucose-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

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Oral D. Y.

Cyprus Journal of Medical Sciences, cilt.11, sa.2, ss.182-187, 2026 (ESCI, TRDizin)

Özet

BACKGROUND/AIMS: The study sought to examine the impact of rosuvastatin and L-arginine, alone or in combination, on hyperglycemia-caused oxidative stress in human umbilical vein endothelial cells (HUVECs). MATERIALS AND METHODS: HUVECs were divided into five groups: (1) control, (2) hyperglycemia, (3) hyperglycemia + rosuvastatin, (4) hyperglycemia + L-arginine, and (5) hyperglycemia + L-arginine + rosuvastatin. The incubation period was 24 hours for all study groups. Cytotoxicity assays were performed for L-arginine and rosuvastatin. After incubation with glucose, L-arginine, and rosuvastatin, malondialdehyde (MDA) level, an oxidative stress marker, and catalase activity were measured. RESULTS: In cytotoxicity tests, the highest non-cytotoxic concentration of L-arginine and rosuvastatin was 12.5 µM. Under hyperglycemic conditions, catalase activity was significantly decreased in HUVECs (p=0.0007 versus control). L-arginine alone (p=0.0016 vs. control) and L-arginine combined with rosuvastatin (p=0.0099 vs. control) resulted in a partial increase in catalase activity. Hyperglycemia caused a significant elevation in MDA levels in HUVECs (p=0.0054 vs. control). L-arginine induced a partial reduction in MDA levels (p=0.03 vs. control), whereas the combination of rosuvastatin and L-arginine restored the altered MDA levels. CONCLUSION: These results indicate that combined incubation with rosuvastatin and L-arginine is more effective in reducing hyperglycemia-induced oxidative stress than administration of either agent alone.