Strawberry production is increasing year by year in Turkey and commercial growing is done mostly using foreign cultivars. In our country, breeding programmes using aromatic local cultivars and some new cultivars such as 'Ebru', 'Sevgi' and 'Kaska' were undertaken. However, the fruits firmness of these cultivars were not found satisfying as commercial ones. Therefore, this research aims to investigate the influence of calcium nitrate applications on the ester profiles of 'Osmanli', 'Camarosa', 'Sevgi', 'Ebru' and 'Kaska'. For this purpose, the effect of various calcium nitrate Ca(NO3)(2) (0 (control), 150, 300 and 450 mL 100 L-1) applications on ester profiles of those cultivars were measured by headspace-solid phase micro extraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS).