Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites

Mercimek H. A., DİNÇER S., Guzeldag G., Ozsavli A., MATYAR F., Arkut A., ...Daha Fazla

BRAZILIAN JOURNAL OF MICROBIOLOGY, cilt.46, sa.1, ss.103-111, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1590/s1517-838246120140026
  • Dergi Adı: BRAZILIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.103-111
  • Anahtar Kelimeler: Pseudomonas sp, biotransformation, nitroaromatic explosives, TNT, TNT RED WATER, NITROAROMATIC COMPOUNDS, CONTAMINATED SOIL, EXPLOSIVE 2,4,6-TRINITROTOLUENE, BIODEGRADATION, TRANSFORMATION, BIOTRANSFORMATION, REMOVAL, STRAIN, MINERALIZATION
  • Çukurova Üniversitesi Adresli: Evet

Özet

Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L-1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL(-1) TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites.