Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites
BRAZILIAN JOURNAL OF MICROBIOLOGY, cilt.46, sa.1, ss.103-111, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 46 Sayı: 1
- Basım Tarihi: 2015
- Doi Numarası: 10.1590/s1517-838246120140026
- Dergi Adı: BRAZILIAN JOURNAL OF MICROBIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.103-111
- Anahtar Kelimeler: Pseudomonas sp, biotransformation, nitroaromatic explosives, TNT, TNT RED WATER, NITROAROMATIC COMPOUNDS, CONTAMINATED SOIL, EXPLOSIVE 2,4,6-TRINITROTOLUENE, BIODEGRADATION, TRANSFORMATION, BIOTRANSFORMATION, REMOVAL, STRAIN, MINERALIZATION
- Çukurova Üniversitesi Adresli: Evet
Özet
Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L-1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL(-1) TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites.